dna methylation

A new single-cell genomics protocol that is potentially transformative for epigenetics research has been developed by scientists in the UK and Belgium. Applying this method, it is now possible to study the epigenome and transcriptome of a single cell at the same time. This novel approach could enhance our understanding of the link between gene expression and DNA methylation in single cells. Also, the knowledge of this relationship may clarify the mechanisms underlying normal development, and changes that occur with [more…]

Recent developments in DNA methylation analysis technologies have made it crucial for researchers to understand which tool is optimal for their epigenetic research. These new methods pose exciting opportunities never before imagined, allowing for epigenetic variation to be connected to phenotypic consequences on a much grander scale and at single-base resolution. In a recent issue of an epigenetics newsletter, The Decoder, scientists at Epigentek discuss the latest progress made in profiling genome-wide and region-specific DNA methylation and offer suggestions on [more…]

Over the past decade significant advances have been made in methylation profiling technology allowing for highly specific and accurate information about the epigenome of various species. Because the 5mC and 5hmC modifications are widespread with possibly different functions, further insight into their distribution is important. Traditional methylation analysis methods such as mass spec, HPLC and TLC allow high accuracy but also require sophisticated equipment, are not high through-put and most importantly are expensive. Likewise more modern applications such as next [more…]