David Esopi
About David Esopi
David Esopi is a researcher specialist in the lab of Dr. Srinivasan Yegnasubramanian at the Johns Hopkins Sidney Kimmel Comprehensive Cancer Center in Baltimore, MD. He has extensive experience with molecular genetics techniques, with particular emphasis on cancer epigenetics.

Collecting & Analyzing Libraries For Chromatin Immunoprecipitation (ChIP)

March 10, 2015 David Esopi

Editor’s Note: At the author’s request on August 20th, 2015, revisions to this article were made following careful consideration. All changes are denoted in red. In the previous post of this ChIP series, we left off with your samples rotating at four degrees overnight, to give the antibodies plenty of time to bind and the beads plenty of time to block.  In this final post, we will finish the assay and provide advice on how to QC and analyze your libraries. [more…]

A Starter Chromatin Immunoprecipitation (ChIP) Protocol

February 10, 2015 David Esopi

Editor’s Note: At the author’s request on August 20th, 2015, revisions to this article were made following careful consideration. All changes are denoted in red. In the first post of this ChIP series, we introduced the reader to the general concept and workflow of chromatin immunoprecipitation (ChIP), and provided advice on planning a ChIP experiment and preparing the necessary reagents.  In this post, we will go through a more detailed protocol that we have used in our lab with success that [more…]

Preparation for Chromatin Immunoprecipitation (ChIP)

February 3, 2015 David Esopi

Editor’s Note: At the author’s request on August 20th, 2015, revisions to this article were made following careful consideration. All changes are denoted in red. Since its introduction in the 1980s, chromatin immunoprecipitation (ChIP) has become one of the most important and powerful techniques in the field of genetics, allowing researchers to characterize a given protein’s binding sites across the genome.  With the advent of next-generation sequencing and whole genome analysis, a single ChIP can deliver gigabytes of information, including not [more…]

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