Accessing Histone PTMs: Insights Into the Methods Used for Their Assessment
This article was first published by EpigenTek. In the early 1960s, Vincent G. Allfrey and his colleagues at the Rockefeller Institute (today’s Rockefeller University) demonstrated by way of C14 labeling the incorporation of methyl and acetyl groups into histones.1 The evidence suggested that these chemical additions succeeded protein synthesis, although the means by which they were incorporated as well as the extent of their biological significance were not fully understood at the time. Allfrey’s pioneering work effectively laid the foundation for histone [more…]
