bisulfite sequencing

The concept of DNA library preparation after bisulfite conversion of DNA was originally introduced by Miura et al. (Nucleic Acids Res. 2012) and commercial products for post-bisulfite library preparation were first developed by Epigentek (EpiNext kit) and Epicentre (EpiGnome kit) in 2013, in order to increase sensitivity and reduce sample loss. The major application of post-bisulfite library preparation is whole genome bisulfite sequencing (WGBS) but, with just a slight change to the protocol, this method could also be used for reduced representation [more…]

A new single-cell bisulfite sequencing (scBS-seq) technique that can advance epigenetic experiments has been developed by researchers from BSRC-funded Babraham Institute and the Wellcome Trust Sanger Institute Single Cell Genomics Centre. Using this powerful technique, all epigenetic marks on the DNA within a single cell can be mapped out. This novel method could enhance our understanding of embryonic development and holds promise for improving clinical applications such as fertility treatments and cancer therapy. It may also reduce the amount of [more…]