Scientists at Stanford University School of Medicine have developed a rapid and sensitive method for integrative epigenomic analysis called assay for transposase-accessible chromatin using sequencing (ATAC-seq).This assay is based on direct in vitro transposition of sequencing adaptors into native chromatin, which captures open chromatin sites using a simple two-step protocol with 500–50,000 cells and reveals the interplay between genomic locations of open chromatin, DNA-binding proteins, individual nucleosomes and chromatin compaction at nucleotide resolution. By using ATAC-seq, the scientists discovered classes of DNA-binding factors that strictly avoided, could tolerate, or tended to overlap with nucleosomes. ATAC-seq maps of human CD4+ T cells from a proband obtained on consecutive days also demonstrated the feasibility of analyzing an individual’s epigenome on a timescale compatible with clinical decision-making.
Source:Learn all about it and read more about their findings here: Buenrostro JD et al., Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nature Methods. Published online 06 October 2013
As skin ages, it may become more sensitive to its environment. A small amount of…
A father’s health before conception may play a larger role in a child’s future biology…
Two people can be the same chronological age, yet their skin may appear to age…
Your skin is often the first place where aging becomes visible. Fine lines, dryness, uneven…
Skincare has been moving steadily away from one-size-fits-all routines and toward measurement-led personalization. One reason…
Consumers often describe a familiar problem: skin that seems to lose firmness, glow, and smoothness…