DNA Deaminase-Based Hydroxymethyl–Seq: Nondestructive and Base-Resolution
The most common epigenetic mark throughout the genome is 5-methylcytosine (5mC). Additionally, 5-hydroxymethylcytosine (5hmC) is also known to play a role in various processes, including embryonic stem cell development and tumorigenesis. There is also an enrichment of 5hmC in human neurons. Accurately quantifying and determining the genomic location of these two different marks is therefore crucial to our understanding of molecular biology and disease. Standard bisulfite sequencing (BS-seq) has been a reliable method for detecting un-methylated and methylated cytosine residues [more…]
