The following histone extraction protocol was written by scientists at Epigentek and is a recommended and optimized procedure for their histone modification assays, successfully utilized by many research labs. Use these easy to follow steps to ensure proper isolation of histone proteins.
For cells (treated and untreated), harvest cells and pellet the cells by centrifugation at 1000 rpm for 5 minutes at 4°C. Resuspend cells in TEB buffer at 107 cells/ml and lyse cells on ice for 10 minutes with gentle stirring. Centrifuge at 3000 rpm for 5 minutes at 4°C. If total volume is less than 2 ml, transfer cell lysates to a 2 ml vial and centrifuge at 10,000 rpm for 1 minute at 4°C. Remove supernatant.
If you’re looking for a commercial kit, Epigentek also offers the EpiQuik Total Histone Extraction Kit for extracting histone proteins in just one hour.
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