How to Choose an Antibody: For Beginners
Antibodies are used extensively in research for procedures, such as western blotting (WB), enzyme-linked immunosorbent assays (ELISA), and immunoprecipitation (IP). The issue of accuracy and reproducibility surrounding the usage of commercially available antibodies for research continues to be a concern within the scientific community. The best way to compile information about an antibody is to review the provided manufacturer datasheet. In fact, some journals will not publish a study that includes an antibody with insufficient information and validation effort. Researchers [more…]